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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants were being documented but detailed nucleotide sequence facts just isn't nonetheless readily available.
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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally includes the epitope recognized with the antibody.
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This information outlines more attainable optimization of mobile and immunoassay parameters to make sure the absolute best outcomes are...
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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining with the blocked antibody compared to the antibody on your own, you can see which staining is precise.
Focus on Facts Cyclin E belongs on the hugely conserved cyclin loved ones, whose customers are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which add for the temporal coordination of each mitotic occasion. Cyclin E kinds a complex with and functions as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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